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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
12/11/2019 |
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Data da última atualização: |
12/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COELHO, S. V. B.; ROSA, S. D. V. F. da; FANTAZZINI, T. B.; SILVA, L. C. |
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Afiliação: |
Stefânia Vilas Boas Coelho, Universidade Federal de Lavras - UFLA; STTELA DELLYZETE VEIGA F DA ROSA, CNPCa; Tatiana Botelho Fantazzini, Universidade Federal de Lavras - UFLA; Luciano Coutinho Silva, Universidade Federal da Paraíba - UFPB. |
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Título: |
Cryopreservation of Coffea canephora Pierre ex A. Froehner seeds: importance of drying rate and moisture content. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Australian Journal of Crop Science, v.13, n. 8, p. 1335-1342, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Seeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation. MenosSeeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzyme... Mostrar Tudo |
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Palavras-Chave: |
Antioxidant enzymes; Drying in silica gel; Ice crystals; Physiological quality; Rapid drying. |
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Thesaurus Nal: |
Recalcitrant seeds. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204558/1/Cryopreservation-of-Coffea-canephora-Pierre.pdf
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Marc: |
LEADER 02404naa a2200229 a 4500
001 2114203
005 2019-11-12
008 2019 bl uuuu u00u1 u #d
100 1 $aCOELHO, S. V. B.
245 $aCryopreservation of Coffea canephora Pierre ex A. Froehner seeds$bimportance of drying rate and moisture content.$h[electronic resource]
260 $c2019
520 $aSeeds of the Coffea canephora species are considered more recalcitrant than those of the Coffea arabica species. They tolerate partial dehydration and they cannot be stored in conventional seed banks at -18ºC because they are sensitive to freezing temperatures. Cryopreservation is a reliable method for storing seeds with these characteristics for long periods. However, preliminary studies are necessary to determine ideal storage conditions. The aim of this study was to investigate the ideal physical and physiological conditions for cryopreservation of Coffea canephora seeds to reduce seed mortality caused by the formation of intracellular ice crystals and to avoid cell damage caused by excessive desiccation. Seeds were subjected to rapid drying in silica gel and slow drying in saturated NaCl solution to moisture contents of 0.20, 0.25, and 0.28 g.g-¹ (dry basis), followed by direct immersion in liquid nitrogen for rapid freezing. Physiological and biochemical analyses were performed to evaluate seed quality before and after cryopreservation. Rapid drying of Coffea canephora seeds to values near 0.20 g.g-1 (db) does not cause reduction in physiological quality. Moisture content of 0.25 g.g-¹ brings about greater survival of Coffea canephora seeds after cryopreservation. Drying rate affects the physiological quality of cryopreserved Coffea canephora seeds, and rapid drying in silica gel is more favorable than slow drying in a saturated NaCl solution. The activity of the enzymes catalase, esterase, glutamic oxaloacetic transaminase, and polyphenol oxidase are indicators of seed quality for Coffea canephora seeds subjected to cryopreservation.
650 $aRecalcitrant seeds
653 $aAntioxidant enzymes
653 $aDrying in silica gel
653 $aIce crystals
653 $aPhysiological quality
653 $aRapid drying
700 1 $aROSA, S. D. V. F. da
700 1 $aFANTAZZINI, T. B.
700 1 $aSILVA, L. C.
773 $tAustralian Journal of Crop Science$gv.13, n. 8, p. 1335-1342, 2019.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 7 | |
| 2. |  | NONNENMACHER, J. L.; BREDA, M.; MATTHIENSEN, A.; MICHELON, W.; DIEFENTHAELER, H. S.; ROGINSKI, A. C.; CAMERA, F. D. M.; SOUZA ROMAN, S. S. Avaliação da toxicidade subcrônica do extrato de microalgas em camundongos. In: SIMPÓSIO SUL DE GESTÃO E CONSERVAÇÃO AMBIENTAL, 9.; SEMANA ALTO URUGUAI DO MEIO AMBIENTE, 25., 2016, Erechim. Ações para a conservação da biodiversidade: anais. Erechim: EdiFAPES, 2016. p. 247-251.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 3. | | NONNENMACHER, J. L.; BREDA, M.; MATTHIENSEN, A.; MICHELON, W.; CANSIAN, R. L.; MIKULSKI, B. S.; ROMAN, S. S. Redução de ganho de peso corporal em camundongos com dieta suplementada com microalgas. In: CONGRESSO DE BIOTECNOLOGIA DA REGIÃO SUL, 1., Lajeado. Cenário atual e perspectivas futuras. Anais... Lajeado: Editora Univates, p. 168, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 4. | | BREDA, M.; NONNENMACHER, J. L.; MICHELON, W.; MATTHIENSEN, A.; CANSIAN, R. L.; ROMAN, S. S. Lise celular mecânico por irradiação ultrassônica e cultivo de microalgas. In: CONGRESSO DE BIOTECNOLOGIA DA REGIÃO SUL, 1., Lajeado. Cenário atual e perspectivas futuras. Anais... Lajeado: Editora Univates, p. 107, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 5. | | BREDA, M.; NONNENMACHER, J. L.; MATTHIENSEN, A.; CANSIAN, R. L.; DIEFENTHAELER, H. S.; PEREIRA, A. A. M.; ROMAN, S. S. Efeito da administração oral de microalgas em camundongos. In: MOSTRA CIENTÍFICA DAS CIÊNCIAS AGRÁRIAS E CIÊNCIAS BIOLÓGICAS, 3., Erechim. Anais... Erechim: URI, p. 278-283, 2016.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 6. | | BREDA, M.; NONNENMACHER, J. L.; KONZEN, A. C.; MATTHIENSEN, A.; CANSIAN, R. L.; DIEFENTHAELER, H. S.; PEREIRA, A. A. M.; ROMAN, S. S.; ZANIN, E. M. Efeito sistêmico do extrato de microalgas em camundongos. In: SIMPÓSIO SUL DE GESTÃO E CONSERVAÇÃO AMBIENTAL, 9.; SEMANA ALTO URUGUAI DO MEIO AMBIENTE, 25., 2016, Erechim. Ações para a conservação da biodiversidade: anais. Erechim: EdiFAPES, 2016. p. 33.36| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| 7. | | BREDA, M.; NONNENMACHER, J. L.; MATTHIENSEN, A.; CANSIAN, R. L.; DIEFENTHAELER, H. S.; ROMAN, S. S. Toxidade aguda do extrato de microalgas em camundongos. In: MOSTRA CIENTÍFICA DAS CIÊNCIAS AGRÁRIAS E CIÊNCIAS BIOLÓGICAS, 3., Erechim. Anais... Erechim: URI, p. 290-291, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Suínos e Aves. |
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| Registros recuperados : 7 | |
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